Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Antibacterial Property of Moringa oleifera Seed Extract: Comparative Study of Crude Extract and Membrane Processed Extract

Munirat A. Idris, Mohammed S. Jami*, Mohd I. Abdul Karim, Parveen Jamal and Suleyman A. Muyibi

Bioenvironmental Engineering Research Centre (BERC), Biotechnology Engineering Department, Faculty of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10 Jalan Gombak, Kuala Lumpur, 50728, Malaysia.

Received on 08 January 2014 and accepted on 24 March 2014

 

ABSTRACT

In this research, the antibacterial property of both crude and membrane processed seed extracts were investigated against Escherichia coli, Pseudosomonas aeruginosa Bacillus subtilis and Staphylococcus aureus. The defatted seeds were prepared with water and the water extract obtained was passed through 0.45?m microfiltration membrane system. The antibacterial activity was done using the agar well diffusion. The effect of concentration of the extracts were determined, the Minimum Inhibitory Concentration (MIC) as well as Minimum Bactericidal Concentration (MBC) was carried out. The result showed that the diameter inhibition zone ranges from 13mm to 30mm and increase in the seed extracts showed a corresponding increase in the inhibition of the bacterial strains. Statistical difference was found (P = 0.002) when the mean of antibacterial activity of the membrane processed seed extract against the bacterial strains was compared with the crude seed extract. Statistical results showed that membrane processed extract was more effective than the crude extract. The results of MIC and MBC values of membrane processed extract seed obtained shows that it was bactericidal against Pseudosomonas aeruginosa, Bacillus subtilis, and bacteriostatic against Escherichia coli Staphylococcus aureus respectively.

Keywords : Moringa oleifera, microfiltration, antibacterial activity, MIC, MBC.