Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Study on Callogenesis and Growth Kinetics of F. deltoidea var. trengganuensis Cell Suspension Culture

TajulAfif Abdullah1*, Azura Amid2, Noor Illi Muhamad Puad2, Parveen Jamal2 and Hafsah Jaafar1

1Department of Biotechnology, Faculty of Agriculture and Biotechnology, Universiti Sultan ZainalAbidin, Kampus Gong Badak, 21300 Kuala Terengganu, Terengganu. 2Bioprocess and Molecular Engineering Research Unit (BPMERU), Department of Biotechnology Engineering, Kuliyyah of Engineering, International Islamic University Malaysia, Jalan Gombak,53100 Kuala Lumpur, Malaysia.

Received on 08 January 2014 and accepted on 24 March 2014

 

ABSTRACT

Active compounds such as secondary metabolite from plants can be produced via its regeneration of organ or callus or from cell suspension culture. Thus, the aim of this study is to establish the protocol for callus regeneration and cell suspension culture of Ficusdeltoidea var. trengganuensis. Callus regeneration study was conducted on solid media consisted of 9 treatments with combination of picloram and 2,4-D ranging from 1.5 ppm to 4.5 ppm. After 4 weeks, the callus were weight under sterilize condition. Treatment consisted of MS + 3 ppm picloram + 3 ppm 2,4-D was found to form the highest weight of callus formation (68.8 ? 21.25 mg). Cell suspension was then established where 4 weeks old of soft and friable callus of F.deltoidea were used as an inoculum at 2% (w/v) in a 100mL of media. About1ml media was collected at 5 days interval to determine its dry cell weight. The cell suspension culture established in this study showed an increase in its dry cell weight (mg/mL) until day 15th where the density of the biomass were observed to decrease at day 20th. The highest specific growth rate (5.49 ? 10-3 h-1) was observed at day 5 to day 10.

Keywords : Callus; cell suspension; dry cell weight (DCW); inoculum density; picloram.