Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Response of Microbial Community Structure to Different Enhanced Oil Recovery Processes in Shengli Oilfield (China)

Lu-Jun Chai1, Fan Zhang1, Yue-Hui She2* and Du-Jie Hou1*

1The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Mechanism, Ministry of Education, China; School of Energy Resources, University of Geosciences (Beijing), Beijing 100083, China. 2College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434023, China.

Received on 12 April 2014 and accepted on 09 May 2014



Aim to study that different enhanced oil recovery processes influence microbial community in the oil reservoir, three different types of samples from Ng3 zone of Shengli oilfield were analyzed: produced water from water-flooded well, from heterogeneous combination flooding well and from microbial enhanced oil recovery process. Based on 454 pyrosequencing of 16S rRNA gene amplicons, microbes clustered into Proteobacteria were dominantly detected in the three samples. However, Dominant microorganisms in three production wells were significantly different at genus level. The majority of reads obtained in three samples were aligned closely to Azospirillum, Pseudomonas, and Thauera, respectively. The microorganisms usually detected in oil reservoirs environments and associated with members of Azospirillum,Pseudomonas, Thauera, Acinetobacter and Petrobacter. Microbial communities in production well after nutrients injection, revealed an activation of the microbes, and some microorganisms were significantly inhibited with a sharp decline in the number. This first investigation of the microbial diversity in oil reservoir with different enhanced oil recovery processes expands substantially the knowledge of the extent of microbial diversity and highlights the complexity of microbial communities.

Keywords : 454 pyrosequencing, microbial community, enhanced oil recovery (EOR).