Journal of Pure and Applied MicrobiologyVol. 7 No. 4

Antimicrobial Resistance and Molecular Typing of Pathogenic Vibrio parahaemolyticus Isolated from Seafood in Shanghai Retail Markets

Danfeng Guo1,2,3, Zhaohuan Zhang1,2,3, Xiaoyang Tang1,2,3, Jingjing Wang1,2,3, YingJie Pan1,2,3 and Yongzhao1,2,3*

1College of Food Science and Technology, Shanghai Ocean University, Shanghai - 201 306, China. 2Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai - 201 306, China. 3Laboratory of Quality, Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai - 201 306, China.

Received on 23 September 2013 and accepted on 06 November 2013

 

ABSTRACT

Vibrio parahaemolyticus is one of the most seafood-borne pathogens in China, which may cause acute gastroenteritis. We aimed to investigate the antimicrobial resistance and genotypic relationship of 41 pathogenic V. parahaemolyticus isolates from 342 seafood samples including 248 shrimp, 86 shellfish and 18 fish. These samples were collected from retail markets in Shanghai during March in 2011 to December in 2012. After preliminary screening in Vibrio chromogenic medium, the assumed isolates were confirmed by amplification of tlh, tdh, trh genes through polymerase chain reaction (PCR) and 41 tdh positive V. parahaemolyticus strains were acquired. Besides, pathogenic strains O3:K6 (Vp2) and ATCC33847 (Vp1) were tested in this study as control. By antimicrobial susceptibility testing?these pathogenic strains were sensitive to chloramphenicol, 14.63% were resistant to amikacin, and there were varying resistance to amoxicilin-clavulanic, gentamicin, ciprofloxacin, levofloxacin. Analysis by enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) revealed that the isolates could be grouped into six patterns. In conclusion, V. parahaemolyticus was commonly found in retail seafood products from Chinese aquatic market, and a few parts were pathogenic and antibiotic resistant strains which may pose a potential threat to human health.

Keywords : Pathogenic V. parahaemolyticus, Antibiotic resistance and ERIC-PCR.