Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Characterization of TEM-, SHV-, CTX- and AmpC-type β-lactamases from Cephalosporin Resistant Escherichia coli Isolates from Northwest of Iran

AlkaHasani1,Akbar Mohammadzadeh1,HosseinSamadiKafil2, Mohammad AhangarzadehRezaee3, Akbar Hasani4* and Mohammad Aghazadeh3

1Infectious and Tropical Diseases Research Center and Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 2Drug Applied Research Center, and Department of Medical Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 3Department of Microbiology, Faculty of Medical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 4Infectious and Tropical Diseases Research Center and Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Received on 29 April 2015 and accepted on 09 June 2015

 

ABSTRACT

Escherichia coli isolates that produce Extended-Spectrum â-Lactamase (ESBL) and AmpC â -lactamases not only cause treatment failure, but usually carry resistance genes for other antibiotics, which limits the choice of therapy. This study focused on the prevalence, detection and comparison of ESBL-type resistance genes (TEM, SHV, and CTX-M) with plasmid-mediated AmpC-producing genes (MOX, CIT, DHA) from E. coli isolates from Northwest of Iran. A total of 146 ceftazidime-resistance Escherichia coli clinical isolates were collected from hospitalized cases of urinary tract infection from Northwest of Iran. Phenotypic and genotypic examination by PCR was done for the presence of ESBL and AmpC genes by PCR. One hundred and twenty eight (87.8%) and 18 (12.3%) isolates were detected possibly as ESBLs and AmpC producers respectively. Of these, 135(92.5%) of the isolates harbored blaCTXM, 105(71.9%) blaTEM , 111(76%) blaSHV, 47(32.2%) CIT, 8(5.5%) MOX and 2 (1.4%) DHA gene. Findings of the present study indicates high rate of resistance to beta-lactam antibiotics due to CTX-M, TEM and SHV genes. Secondly, in 32% of AmpC isolates CIT genes was detected, which indicates high prevalence of this gene. This findings shows importance of developing local stewardship program for antibiotic consumption and screening isolates for the presence of ESBL and AmpC genes.

Keywords : Escherichia coli, ESBL, AmpC, Resistance, Phenotypic, Genotypic.