Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Rapid Detection of GanodermaDisease of Coconut by using ITS-PCR and Assessment of Inhibition Effect of Various Control Measures by Fungicides and Bioagents

J. Raju1, S. T. Naik2, S. Priti5, V. Suryanarayana4, V. I. Benagi2, Jones Nirmalanath3 and Madhu S. Giri2

1Department of Plant Pathology, University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka 577201, India. 2Department of Plant Pathology, University of Agricultural Sciences, Dharwad, Karnataka 580 005, India. 3Department of Agricultural Microbiology, University of Agricultural Sciences, Dharwad, Karnataka 580 005, India. 4Department of Plant Pathology, College of Forestry, University of Agricultural Sciences, Dharwad, Karnataka 580 005, India. 5Department of Plant Pathology, Central horticultural Experiment Station, Indian Institute of Horticulture Research, Chettalli, Kodagu, Karnataka 571248, India.

Received on 08 August 2015 and accepted on 09 October 2015

 

ABSTRACT

Two biochemical and molecular methods have been applied for detecting the Ganoderma, a basidiomycete fungi causing basal stem rot disease (BSR) of coconutin the early stage itself. Leaf, stem/bark and root extracts recorded highest optical density values than their corresponding healthy tissues. Increased transmittance values observed from the extracts of diseased leaf, stem, bark and root extracts compared healthy tissues. For polymerase chain reaction (PCR) test, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 160 bp in size is used for early detection. Different systemic, non-systemic and bioagents were screened against Ganoderma isolates for in vitro management of the disease. Among the non-systemic fungicides tried in vitro, captan, mancozeb and thiram at 0.2 per cent completely inhibited the growth of the fungus. In case of systemic fungicides trideomarphm, hexaconzole and carbendazim at 0.1 per cent recorded highest per cent inhibition of mycelium growth. Dual culture study carried out with three different species of Trichodermarevealed that T. harzianumand T. koningiiwere found effective.

Keywords : Biochemical, Molecular, diagnosis, Ganoderma, Bioagents, fungicides