Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Development of Antibodies in Broiler Chickens Immunized with Adjuvanted Eimeria tenella Sporozoites

Saravanan Subramanian1*, Palanivel Kondan Muthusamy1, Selvaraju Ganapathy1 and Srinivasan Palani2

1Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Namakkal - 637 002, India. 2Associate Professor, Poultry Disease Diagnosis and Surveillance Laboratory, Veterinary, India.

Received on 20 April 2015 and accepted on 10 June 2015

 

ABSTRACT

Humoral immune response elicited by administration of purified adjuvanted E. tenella sporozoites by S/C in neck region of broiler chickens in different age groups was assessed by a developed indirect ELISA. The chickens of group (T2) immunized with 25 ?g of antigen with Freund?s Complete adjuvant (FCA) on day 2 and a booster vaccination without adjuvant on 18th day of age inducted peak IgG levels (0.616?0.022) at six weeks of age post immunization (PI) with a decline (0.394?0.012) at 7 weeks of age. A protective immune response against caecal coccidiosis could therefore be achieved by an immunization with adjuvanted E. tenella sporozoites in chickens of less than a week old, followed by a booster without adjuvant. Further, the performance of immunized chickens as indicated by mean weight gain (302.5?13.834g), mean lesion score (1.33?0.21) and mean oocyst output per gram (OPG) of faeces (53.84?19.09 x103) was found to be superior to the non- adjuvant vaccinated group and unimmunized infected control, however, the mean lesion score and OPG of faeces were inferior to that of medicated group.

Keywords : E. tenella sporozoites, FCA adjuvant, IgG levels, weight gain, lesion score.