Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Trichoderma and Bacillus as Biocontrol Agents against Fusarium in Rice

Kalaivani Nadarajah1* and Hamdia Z. Ali2

1School of Environmental and Natural ResourcesSciences, Faculty of Science and Technology, UniversitiKebangsaan Malaysia, 43000 UKM Bangi, Selangor, Malaysia. 2School of Biosciences and Biotechnology, Faculty of Science and Technology, UniversitiKebangsaan Malaysia, 43000 UKM Bangi, Selangor, Malaysia.

Received on 28 April 2015 and accepted on 11 June 2015

 

ABSTRACT

This study was conducted to determine the effectiveness of Trichoderma and Bacillus isolates used individually or in combination in reducing the disease incidence of FusariumspF2 in rice. The antagonistic activity of twenty-two Trichodermaisolateswas evaluated individually or in combination with Bacillus subtilisUKM1 against Fusariumsp F2via the dual culture technique. The dual culture technique showed highest reduction in radial growth of pathogen by isolates T2, T3, T4, T5, T7, T8, T9, T11 and T21. This was then followed bygreenhouse experiments that evaluatedfour parameters: Pre and Post emergence of seedlings, disease incidenceand disease severityon treated and non treated MR219 rice varieties that were inoculated withFusariumsp F2.The results of this experiment showed that rice plants treated with biocontrolagents showed significant reduction of pathogen (pd? 0.05). The greenhouse experiments further selected isolatesT2, T4 and T21 as the best candidates based on disease severity scores. However when co-inoculatedwithB. subtilis UKM1isolatesT2, T7, T8, T9 andT18resulted in higher reduction of disease incidence (22, 11, 11,22 and 22 %) and severity (8.67, 6.7,4.3, 11 and 9 %) 60 days post transplantation.

Keywords : Fusariumsp, biocontrol,Trichoderma sp., Bacillus substilis, rice, co-inoculated.