Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Evaluation of Antifungal Activity of Lactic Acid Bacteria against Aspergillus flavus

M. Ashwini1, Mahesh V. Kamble1, Smita R. Babar1, Shamrao Jahagirdar2, K. S. Jagdeesh3 and J. A. Mulla4

1AICRP on EAAI (Bioconversion Technology), Main Agriculture Research Station, University of Agricultural Sciences, Dharwad-580005, Karnataka, India. 2Department of Plant Pathology, UAS, Dharwad, India. 3Department of Agri. Microbiology, UAS, Dharwad, India. 4Department of Animal Science, UAS, Dharwad, India.

Received on 12 March 2015 and accepted on 26 April 2015

 

ABSTRACT

Lactic acid bacteria (LAB) are considered as promising natural biological antagonists for mycotoxigenic fungi that contaminate various food commodities. A total 176 LAB isolated from different sources were screened against A. flavus by employing Dual culture assay method. Out of 176, two isolates viz., LS-36 and LS- 44 showed highest inhibition of the toxicogenic fungi under in vitro conditions as well as reduction of fungal mycelium under in vivo conditions. Based on our previous studies, 16S rRNA based molecular characterization of these two isolates showed sequence homology of 99 to 100% towards Lactobacillus brevis.

Keywords : Lactic Acid Bacteria, Aspergillus flavus, Antifungal activity, 16S rRNA.