Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Statistical Optimization of b-1,3 Glucan production from the Novel Strain Bacillus cereus LVK13 (KC 898956)

Lakshminarayanan Vijayakumar1*, Ponnuswamy Ramalingam2, Sundaramurthi Pavithra1 and Rengaraju Balakrishnaraja1

1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam - 638401, Tamil Nadu, India. 2Department of Biotechnology, Kumaraguru College of Technology, Coimbatore - 641006, Tamil Nadu, India .

Received on 18 October 2015 and accepted on 06 December 2015

 

ABSTRACT

b-1,3 glucan, an Exopolysaccharide (EPS) that has enormous industrial applications can be produced commercially through microbial fermentations. The huge market demand necessitates the industries to search for new sources or to implement new strategies to maximize the yield of glucan. Hence, the present study focused on optimization of components of the culture medium and process variables to increase ?-1,3 glucan production from Bacillus cereus LVK13 (KC 898956) using statistical tools. The essential nutrients that influence ?-glucan production was screened using Plackett-Burman Design (PBD). Further, the three-screened components out of the eleven were analyzed with central composite design (CCD). The optimum concentrations of carbon, nitrogen and manganese sources were found to be 80, 1.5 and 0.03 g/l. Second order polynomial regression model accurately showed interpretation of experimental data with an R2 value of 0.9563, Adjusted (Adj) R2, Predicted (Pred) R2 and F values of 0.9474, 0.9329 and 182.69 respectively.

Keywords : ?-glucan, Sucrose, Bacillus cereus LVK13, Optimization, RSM.