Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Isolation and Characterization of Endophytes from Recalcitrant Plant Species

Jasleen Kaur, Ashish Vyas and Chayanika Putatunda*

Department of Microbiology, School of Biosciences and Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Phagwara, Punjab, India.

Received on 21 June 2015 and accepted on 15 August 2015

 

ABSTRACT

Endophytes play crucial role in the life of the plants. Endophytes present in recalcitrant plants are of great value because such plant which harbor endophytes are more efficient in having antimicrobial activity, higher rates of nitrogen fixation and production of various useful biochemical products. In the present study, isolation of endophytes from recalcitrant plants was done followed by characterization. A total of seven bacterial endophytes and seven fungal endophytes were obtained. The endophytic bacterial isolate EBML showed good antibacterial property against E.coli NAIMCC 008483 and Bacillus subtilis NAIMCC and endophytic fungal isolate EFMR obtained showed good antibacterial property against and E. coli NAIMCC 008483, Bacillus subtilis NAIMCC 01233 and Klebsiella NAIMCC 00702. The endophytic isolates also showed good cellulase production in the range of 0.10-0.19 mg/ml/min in case of bacterial endophytes and in the range 0.19 ? 0.21 mg/ml/min in case of fungal endophytes. pH characterization was found to be optimum at 7 for cellulase production for bacterial and fungal isolates. On the basis of 16srDNA and 28srDNA sequencing bacterial endophytic isolates were identified as Bacillus sp. and Trichoderma sp. respectively.

Keywords : Endophytes; Recalcitrant plant; Cellulases; Antimicrobial activity.