Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Compatibility of Biocontrol Bacteria with Phyto-Extracts

D. G. Panpatte1*, H. N. Shelat2 and Y. K. Jhala3

*1Ph. D. Scholar, 2Associate Research Scientist, 3Assistant Professor Department of Agricultural Microbiology, Anand Agricultural University, Anand-388 110, India.

Received on 13 June 2015 and accepted on 19 September 2015

 

ABSTRACT

Biocontrol agents and phyto-extracts (botanicals) have a great promise as preventive measure against phyto-pathogens and till date negligible research attention has been to evaluate compatibility of these two bicontrol agents. In present investigation, compatibility of phyto-extracts viz. neem extract, besharam extract and mustard cake extract with native biocontrol agents (Providencia vermicola AAU PR1, Pseudomonas putida AAU PR2 and Pseudomonas fluorescens AAU PR3) was checked. All the cultures were found to be compatible with phyto-extracts of neem, besharam and mustard cake on solid agar medium showing no zone of inhibition. The isolates were further confirmed to be compatible with phyto-extract in liquid assay wherein there was no reduction in optical density of cultures was recorded. Moreover, neem, besharam and mustard cake extract up to 10 % concentration showed to increase the growth of all the three tested microbial strains. The results of the present investigation reveal the scope of simultaneous application of biocontrol bacteria and phyto-extract to get benefit of wider range of biocontrol naturally.

Keywords : phyto-extract, botanicals, biocontrol,