Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Molecular Identification of Salt Tolerant Endophytic Bacteria from Kutch, India by Sequencing of the 16S rRNA coding gene

Roshani A. Bhadania*, B.A. Golakiya and D.L. Akbari

Department of Biotechnology, Junagadh Agricultural University, Junagadh, Gujarat, India.

Received on 06 May 2015 and accepted on 19 July 2015

 

ABSTRACT

The importance of plant growth promoting rhizobacteria in growth promotion and their ability to elicit ?induced systemic tolerance? against abiotic stresses has been documented. However, the performance of these microbes under various abiotic stresses especially saline-sodic conditions will be of great importance in the current agricultural scenario. In present study salt tolerant endophytic bacteria isolated from four wild Malvaceae plant native to the different area of Kutch (Abuliton indicum, Senra incana, Sida sp., wild cotton) and identified, through partial sequencing of the 16S rRNA encoding gene. The sequence of 17 isolates deposited in NCBI and compared to sequences- 29.41% belonged to the genus Staphylococcus; 17.64% to Bacillus; 11.76% both to Providencia and Microvirga, whereas 5.88% were Alcaligens. Results obtained through sequence analysis revealed high genetic diversity across the isolates.

Keywords : Malvaceae plant, salt tolerant endophytic bacteria, 16S rRNA Sequencing, NCBI, BLASTn