Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Identification of FPV by PCR amplification of p4b gene in infected cell culture and Chorioallantoic membrane

R. K. Verma1, R. K. Joshi2 and Shubhra Shukla3

1Department of Veterinary Teaching Veterinary Clinical Complex (T.V.C.C), College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad (U.P.), India. 2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry N.D. University of agriculture and Technology Kumarganj- 224229, Faizabad (U.P.), India. 3Department of Biosciences, Integral University, Lucknow (U.P.) 226026, India.

Received on 20 May 2015 and accepted on 11 August 2015

 

ABSTRACT

Five local fowl were brought for postmortem examination with a history of sudden death in the area of Kumarganj, Faizabad (U.P.). The gross examination of birds revealed multiple light whitish nodules around the eye, on the skin at the level of hock joint, on the anterior part of tracheal mucosa, congested lung and pallor liver. Impression smears from nodules revealed numerous heterophils, red blood cells, necrotic epithelial cells and bacterial colonies. Histopathological examination of nodules revealed eosinophilic intracytoplasmic inclusions (Bollinger bodies) in keratinocytes, epidermal hyperplasia and necrosis with ballooning degeneration, and bacterial colonies. The virus was isolated and infection was produced on both chorioallantoic membrane and BGM-70. Polymerase chain reaction was carried out and primer set designed from the 4b core protein gene of fowl poxvirus revealed amplification at 576bp.

Keywords : Avianpox virus, Polymerase chain reaction (PCR), BGM-70, Chorioallantoic membrane.