Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Antibacterial Activities of Selenium and Selenium Nano-particles (Products from Lactobacillus acidophilus) on Nosocomial Strains Resistant to Antibiotics

Maryam Beladi1, Abbas Akhavan Sepahi1*, Sedigheh Mehrabian1, Akbar Esmaeili2 and Fariba Sharifnia3

1Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran. 2Department of Chemical Engineering, Islamic Azad University, Tehran North Branch, PO Box 19585/936, Tehran, Iran. 3Department of Biology, Islamic Azad University, Tehran North Branch, Tehran, Iran.

Received on 14 September 2015 and accepted on 16 November 2015

 

ABSTRACT

Nosocomial infections are the major concern in the world. Today, with the emergence of antibiotic resistance among pathogenic bacteria, treatment of this category of infectious diseases is facing numerous problems. The present study investigation of antibacterial activities selenium and selenium nano-particles (synthesized by Lactobacillus acidophilus). Lactobacillus acidophilus and Lactobacillus plantarum were cultured in (MRS) broth medium. The 3-5% of inoculums culture and 10 ml of stock solution of selenium (NaHSeO3) separately were added into two Erlenmeyer flasks containing (MRS) broth medium. The nano-particles were analyzed using scanning electron microscopy (SEM). In this study, the urine, blood, sputum and wound specimens of patients were collected. The isolates were identified and confirmed using the standard microbiological method and 50 antibiotic resistance strains from 436 samples were selected (80% Escherichia coli and 20% Acinetobacter). Antibacterial activities selenium and selenium nanoparticles on isolated bacterial strains were evaluated by disk diffusion and serial broth dilution methods to determine (MIC) and (MBC). The SEM results showed that L.acidophilus nano-particles are smaller than of L. plantarum, therefore it would be preferred for further investigation. The antibacterial activities of the selenium and selenium nano-particles demonstrated to be effective on 32% and 56% of the strains studied, respectively.

Keywords : Nosocomial infections; antibiotics resistant bacteria; Lactobacillus acidophilus; Lactobacillus plantarum; Selenium; Selenium nano-particles.