Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Conditions for the Cultivation of New Bacillus Bacteria being Micro Bioproduct Producers

A.M. Asaturova, A.I. Homyak, N.S. Tomashevich, M.D. Pavlova, N.A. Zhevnova, V.M. Dubyaga, A.Ye. Kozitsin, T.M. Sidorova, V.D. Nadykta and V.Ya. Ismailov

All-Russian Research Institute of Biological Plant Protection.

Received on 16 October 2015 and accepted on 17 November 2015

ABSTRACT

Optimal conditions for cultivation of B. subtilis BZR 336g and B. subtilis BZR 517 strains have been found in the course of the study: temperature, pH, carbon and nitrogen nutrition sources. As a result of the research some original samples of optimized culture media were obtained to produce liquid agent cultures (LAC) with an optimal number of colony-forming units combined with a high anti-fungal activity.

Keywords : Bacteria-antagonists, Bacillus subtilis, Cultivation conditions, Temperature, pH, sources of nutrition.