Journal of Pure and Applied MicrobiologyVol. 9 No. 4

Influence of Antifungal Compound Synthesis by Anabaena against Sheatblight and Blast of Rice

Soheila Afshari Azad, Farzaneh Hosseini*, Parisa Nejatkhah Manavi and Abbas Akhavansepahi

Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran. Iran.

Received on 27 September 2015 and accepted on 14 November 2015

 

ABSTRACT

Cyanobacteria are one of the most diverse groups of prokaryotae which are distributed around the world. They are producing a variety of secondary metabolites which are composed of various biological effects. In this study, the cyanobacteria Anabaena was separated and purified from Sorkhankol area in the central part of Anzali wetland. Diagnoses were done with valid keys and 16srRNA molecular analysis. Methanol extracts and acetone extracts of Anabaena have been examined against Pyricularia oryzae and Rhizoctonia solani fungus (the factor of Blast and Sheath blight of rice diseases). Antifungal properties of extracts on Pyricularia oryzae and Rhizoctonia solani have been determined through Park Method. The results indicated that the diameter of inhibition zone for methanol extracts of Pyricularia oryzae and Rhizoctonia solani were 9 mm and16 mm respectively, the diameter of inhibition zone for acetone extract of both Pyricularia oryzae and Rhizoctonia solani were 8 mm. Furthermore, minimum inhibitory concentration of growth (MIC) for methanole extract were 32 ?g/ml and 16 ?g /ml for Rhizoctonia solani and Pyricularia oryzae minimum inhibitory concentration of growth (MIC) for acetone extract were 32 ?g /ml for both Rhizoctonia solani and Pyricularia oryzae. phytochemical tests for methanole extracts were conducted in this study.

Keywords : Sorkhankol, cyanobacteria, Pyricularia oryzae, Rhizoctonia solani.