Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Detection of Extended Spectrum Beta Lactamase Among Enterobacteriaceae from Various Clinical Samples in a Tertiary Care Hospital

Anjana Telkar1, C. Baragundi Mahesh2 and M.R. Navya1

1J.J.M. Medical college, Davangere, India. 2S.N.M.C.Bagalkot, India.

Received on 11 July 2015 and accepted on 21 August 2015



ESBL producing gram-negative organisms have posed a significant threat to hospitalized patients due to their hydrolyzing activity against extended spectrum cephalosporins often employed in the treatment of hospital-acquired infections. They are increasing rapidly and becoming a major problem in the area of infectious diseases. A delay in appropriate therapy can cause severe complication. The study was conducted on 75 isolates of enterobacteriaceae from various clinical samples. ESBL detection was done according to CLSI 2014 guidelines by Screening test (Disc diffusion method) and Confirmatory test (Combined Disc diffusion method). The most common isolate was E.coli (65.33%) followed by Klebsiella spp (26.66%) and proteus spp (8%). 45(60%) isolates were screening test positive and 30 (40%) were confirmatory test positive in ESBL detection tests according to CLSI guidelines. Maximum ESBL production was shown by Klebsiella spp (50%) followed by E.coli (38.77%). The prevalence of ESBL was found to be high in our hospital which cannot be ignored. We recommend disc diffusion test using multiple antibiotics in all microbiology units as a routine screening test and if possible the confirmatory test. The detection of ESBL producing strains will also help to establish and implement a strict infection control policy to stop the spread of ESBL producing organisms and in turn will reduce the morbidity and mortality of the patients.

Keywords : ESBL, Enterobacteriaceae, CLSI.