Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Molecular Detection of Metallo-Beta-Lactamase genes in Clinical Isolates of Acinetobacter baumannii

Hossein Goudarzi1, Elnaz Sadat Mirsamadi*1, Zohreh Ghalavand1, Mojdeh Hakemi Vala1, Hamed Mirjalali2, 3, Ali Hashemi1 and Ehsan Ghasemi1

1Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2Foodborne and Waterborne Diseases Research Center, Research institute for Gastroentrology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3Gastroenterology and Liver Diseases Research Center, Research institute for Gastroentrology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Received on 10 July 2015 and accepted on 09 September 2015

 

ABSTRACT

Acinetobacter baumannii is an opportunistic pathogen causing infections in hospitalized patients, particularly in intensive care units (ICUs). The treatment of these infections is one of the most important concerns of physicians due to multidrug-resistant. The b-lactam antimicrobial drugs are choice treatment of infections caused by A. baumannii. The specimens included CSF (cerebrospinal fluid), wound, sputum, urine, BAL (Broncho Alveolar Lavage), blood and so on were taken from 128 patients who were hospitalized in several wards. The antimicrobial susceptibility test was carried out using kirby-bauer disc diffusion method for Piperacillin-Tazobactam, Ceftriaxon, Ceftazidime, Cefepime, Imipenem, Doripenem, Ertapenem, Cefotaxime, Ampicillin-Sulbactam antibiotics for all isolates. DNA of isolates was extracted and primers were designed and as well as amplification was carried out for blaIMP, blaVIM, blaNDM1 genes. The resistance to all discs used in this study was seen in all isolates. The Highest and the lowest rate of resistance were seen in Piperacillin-tazobactam and Ampicillin-sulbactam, 100% and 21% isolates, respectively. The results of PCR of blaIMP, blaVIM and blaNDM genes were as follows: blaIMP 5/128 (3.9%), blaVIM 9/128(7.03%) and blaNDM 0/128(0%). The highest sensitivity to antibiotics group was seen in Ampicillin-Sulbactam groups and also blaVIM was the most prevalent gene of resistance among Metallo-Beta-Lactamase genes in Acinetobacter baumannii isolates.

Keywords : Iran, Acinetobacter baumannii, Polymerase Chain Reaction (PCR), Metallo-Beta-Lactamase.