Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

High Level Amylase Production by Pseudomonas stutzeri ML-18; Isolation, Identification and Media Optimization in a Fermentation System using Response Surface Methodology (RSM)

Aboozar Kazemi1, Mojtaba Lorpour1, Ahmad Gholami1,2, Seyed Reza Karimi-Ghavamabadolya1,2, Azam Safari1, Kavous Solhjoo3 and Younes Ghasemi1,2*

1Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. 3Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.

Received on 20 January 2014 and accepted on 10 February 2014

 

ABSTRACT

The bacterium Pseudomonas stutzeri ML-18 was isolated from the kitchen waste water of a sweet shop. The production of amylase by this bacterium was investigated and optimized using statistical approaches (Response Surface Methodology (RSM); Box?Behnken design). The optimized medium was obtained with these conditions; (% w/v) KH2PO4 0.1, Na2PO4 0.25, NaCl 0.1, KNO3 0.1, CaCl2 0.005, tryptone 0.2, MgSO4.7H2O 0.005, starch 1 and pH 8. The amylase activity in optimized medium was 243.614 U/ml. furthermore, high level of amylase production (245.612 U/ml) was observed by using fermentor system.

Keywords : Amylase, Pseudomonas, RSM, optimization.