Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Optimization of Culture Conditions and Biosurfactant Production from Bacillus altitudinis (BMSed II)

D. Das, S.B. Mohapatra* and P. Ray

Department of Microbiology, College of Basic Science and Humanities, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India.

Received on 09 March 2015 and accepted on 15 April 2015

 

ABSTRACT

Biosurfactants are amphiphilic compounds produced on living surfaces, mainly on surfaces of microorganisms or secreted extracellularly. Out of twenty isolates obtained from Bhitarkanika mangrove forest, five were positive for biosurfactant production. Biosurfactant production (mg/ml) and surface tension reduction (mN/m) on different days of incubation of all the five isolates when considered, only one isolate (BMSed II) was found to produce the maximum biosurfactant & highest surface tension reduction on 7 days of incubation in comparison to others. On optimization of different parameters (2% inoculum, 2% glucose, 37?C temperature and pH ? 8) on potent isolate BMSed II was found to be suitable for maximum biosurfactant production of 2.14g/l. The 16S rDNA gene sequence of (BMSed II) was submitted to NCBI gene bank and found similar to Bacillus altitudinis (Accession number KT005170) by phylogenetic analysis. The biosurfactant produced by Bacillus altitudinis was characterized as a lipopeptide using Thin-layer chromatography (TLC), Fourier transform infrared spectroscopy (FTIR) and H1 Nuclear magnetic resonance (NMR) analysis.

Keywords : Biosurfactant, Bacillus altitudinis, lipopeptide, TLC, FTIR, NMR.