Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Activity Profile of Defense Related Enzymes in Chickpea (Cicer arietinum L.) against Fusarium Wilt (Fusarium oxysporum f. sp. ciceris) at Different Growth Stages

U.S. Dalvi*, R.M. Naik, V.P. Chimote and P.N. Harer

Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri - 413 722 India.

Received on 02 August 2015 and accepted on 10 September 2015

 

ABSTRACT

Twenty four promising chickpea genotypes with known wilt resistant and susceptible checks were evaluated for phenol content and activity profile of ?-1,3 glucanase, chitinase, phenylalanine ammonia lyase and polyphenol oxidase at pre and post infection stages under wilt sick soil with simultaneous recording of wilt incidence score . The activity of all these enzymes was significantly increased at post infection stage of growth in wilt resistant chickpea genotypes as compared with wilt susceptible genotypes suggesting induction of the defense response. Amongst twenty four chickpea genotypes when screened in wilt sick soil along with wilt resistant and susceptible checks , three genotypes viz., GL29285(83.3%), PBG5(26.8%) and GL27014 (81.7%) were categorized as wilt susceptible, while twenty one genotypes were wilt tolerant having higher activity profile of defense related enzymes with less than 10% wilt incidence. Among the twenty one chickpea genotypes screened under wilt sick soil BCP 2010-1, JG24 and JG2001-4 recorded significantly higher increase in activity profile of all the four enzymes and total phenolics content. The same three genotypes also recorded low wilt incidence in the field.

Keywords : Wilt, Chickpea, Genotypes, Enzyme, Defense.