Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

An Improved Method for Purification of Marine Cyanobacteria Isolated from the Gulf of Thailand

Nipat Maneechote and Saisamorn Lumyong*

Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.

Received on 15 September 2015 and accepted on 18 October 2015

 

ABSTRACT

Thirty five marine cyanobacteria isolates obtained from water samples collected from the Gulf of Thailand of the Chumpon and Chonburi provinces. They were purified by homogenizing, washing, lysozyme, antibiotics and dilution. Successful purifications were checked by morphological characterization (plate culture and scanning electron microscope) and molecular methods (Multiplex randomly amplified polymorphic DNA and 16S rRNA gene). This study presents the results of axenic cyanobacteria were obtained on ASNIII agar plates and scanning electron microscopy. The DNA fingerprints of all axenic marine cyanobacteria were divided into eight clusters and identified using 16S rRNA gene sequence correlatively close to Geitlerinema sp., Leptolyngbya valdenosa, Leptolyngbya sp., Synechococcus sp., Pseudoanabaena limnetica (Oscillatoria limnetica) and Oscillatoriales cyanobacterium.

Keywords : Cyanobacterial diversity, Molecular identification, Phylogenetic trees, Systematic purification.