Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Isolation, Characterization and Identification of Lipolytic Thermophiles with Methanol Tolerance from Domestic Compost

Syifa F. Syihab, Fida Madayanti and Akhmaloka*

Biochemistry Research Group, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung. Indonesia.

Received on 10 Augsut 2015 and accepted on 03 September 2015

 

ABSTRACT

Five thermophilic bacteria with lipolitic activity were isolated from domestic compost namely AL17, AL59, AL64, AL89, and AL96. All of the isolates showed rod shape and gram negative microbes. The lipase activity among the isolates are varies when grown in medium with pH 7.0 and pH 9.0. AL17 and AL89 isolates showed best activity in medium with pH 7.0. Meanwhile AL64 and AL96 showed less activity in both type of the media, in addition AL59 was found to be mostly active when cultivated in pH 9.0. Methanol tolerant properties of the isolates was investigated through addition of 3% methanol in medium. Growth rate measurement of the isolates showed that AL96 was better methanol tolerant properties compared to others. The 16S rRNAs genes of all five bacteria have been sequenced and deposited to GenBank. Phylogenetic analysis of the 16S rRNA genes showed that four isolates close to genus Pseudoxanthomonas, while the other was highly similar to Brevibacillus thermoruber.

Keywords : Lipase, 16S rRNA, Methanol Tolerant Bacteria.