Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Applicability of Genotypic and Phenotypic Methods to the Assessment of the SKEO Effect on MexXY-OprM and MexEF-OprN Efflux Pumps in MDR and Clinical Isolates of Pseudomonas aeruginosa

Iman Islamieh Davood1, Esmaeili Davood2, Goudarzi Hossein3* and Moradi Fatemeh4

1Ph.D. Student in Medical Bacteriology, International Branch, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2Department of Microbiology, Baqiyatallah University Medical of Sciences, Iran. 3Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4M.Sc. Student in Medical Microbiology, Department of Microbiology, Baqiyatallah University Medical of Sciences, Tehran, Iran.

Received on 11 August 2015 and accepted on 14 October 2015

 

ABSTRACT

In this study to be tried that check out the effect of Satureja khuzistanica essential oil (SKEO) against mexXY-oprM and mexEF-oprN efflux pumps genes expression in MDR and clinical isolates of Pseudomonas aeruginosa. For notice of the genes expression amount before and after of confronting with SKEO, genotyping and phenotyping methods were used. In phenotyping method was applied MIC technique with specific antibiotic markers (Norfloxacin and Imipenem for MexEF-OprN and Gentamicin for MexXY-OprM). In genotyping method was used of RT-PCR technique. The amount of SKEO,s MIC compared to antibiotics were much less. The MIC of antibiotic markers and intensity of bands on gel electrophoresis after confronting decreased. Reduce in MIC of antibiotic markers and as well as in the bands intensity show that SKEO decreased expression pumps and both methods confirm each other. Therefore, SKEO could be an important factor in the reduction of antibiotic resistance in the P. aeruginosa isolates. According to these results, we expect be applied SKEO as a supplement therapy in future infections.

Keywords : Genotypic and phenotypic methods, SKEO Effect, Pseudomonas aeuroginosa.