Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Characterization of Xylanase Protein Sequences of Bacillus cereus: An In-Silico Study

Asish Mandal

Post Graduate Department of Botany, Ramananda College, Bishnupur, Bankura, India.

Received on 21 March 2015 and accepted on 15 May 2015



The use of hemicellulose enzymes is recently gained considerable interest in different industries. The challenges in incorporating the enzyme to the industrial sectors are to have an active and stable form of it at high temperature and wide range of pH conditions. In this study xylanase protein sequences of 10 strains of Bacillus cereus were retrieved from the database of NCBI. These sequences were analysed by computational methods for different physical and chemical properties, multiple sequence alignment, phylogenetic tree construction to predict the final motif and the evolutionary relationship among them. The multiple sequence alignment of these xylanase protein sequences showed conserved regions at different stretches with maximum homology from amino acid residues 123-129, 147-154, 178-182 which could be used for designing probes specific for xylanase producing strains.

Keywords : Bacillus cereus, xylanases, motif, protein designing.