Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Streptomyces brollosae sp. nov., NEAE-115, A Novel L-Asparaginase Producing Actinomycete Isolated from Brollos Lake at the Mediterranean Coast of Egypt

Noura El-Ahmady El-Naggar1* and Hassan Moawad2

1Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria, Egypt. 2Department of Agricultural Microbiology at National Research Center, Cairo, Egypt.

Received on 15 March 2015 and accepted on 27 May 2015

 

ABSTRACT

An actinomycetes strain producing L-asparaginase, strain NEAE-115, was isolated from a soil sample collected from Brollos Lake at the Mediterranean coast of Egypt. The identification of this strain was established using the morphological, cultural, physiological, biochemical properties and 16S rRNA sequence. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence indicated that the strain NEAE-115 belongs to the genus Streptomyces and consistently falls into a clade together with Streptomyces niveoruber strain NRRL B-2724, Streptomyces polychromogenes strain EAAG77, Streptomyces nashvillensis strain 1534, Streptomyces roseoviridis strain JS-9 and Streptomyces venezuelae strain JS-11. 16S rRNA gene sequence similarities between NEAE-115 and above mentioned type strains were between 80 and 81%. The comparative study on the basis of morphological, physiological and biochemical characteristics of the strain NEAE-115 in relation to its closest phylogenetic neighbours of the genus Streptomyces revealed that the strain NEAE-115 represents a novel species of the genus Streptomyces, for which the name Streptomyces brollosae NEAE-115 is proposed and its sequencing product was deposited in the GenBank database under accession number KJ410229.

Keywords : Streptomyces brollosae NEAE-115, 16S rRNA sequences analysis, scanning electron microscope, phenotypic characteristics.