Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Proteome and Quality Analysis of Potato Tubers Infected by Pectobacterium carotovorum sub sp. carotovorum (PP 4)

V. Ponvizhi Ramya*, S. Gunasekaran and M. Senthil Kumar

Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore - 641 003, India.

Received on 08 June 2015 and accepted on 24 September 2015

 

ABSTRACT

Pectobacterium carotovorum subsp. carotovorum is an important soft rot pathogen that infects many vegetables in India. Healthy potato, naturally infected potato and artificially pathogen inoculated potato on third day were evaluated for differential protein expression, Polyphenoloxidase activity, total phenolic compound, titratable acidity, pH, soluble solids content, reducing sugars, colour and firmness. Totally 6 differentially expressed products were obtained in two dimensional gel electrophoresis. Among that spots numbered 1,2,5,6 were up regulated and 3, 4 down regulated indicating pathogenesis related proteins and disease resistant inhibitor proteins respectively in both naturally infected and P. carotovorum subsp. carotovorum (PP 4) inoculated when compared with the healthy tissue. It requires more evidence to prove their functions in future. Increase in reducing sugars, total soluble solids, polyphenoloxidase activity, total phenolic compound and decrease in firmness and colour in infected potato than the healthy exactly indicates the quality changes of potato by spoilage pathogens. To our knowledge the present investigation is the detailed report on the effects of P. carotovorum sub sp. carotovorum on the quality of post harvest potatoes.

Keywords : Differential protein expression, Pectobacterium, Potato, Soft rot, Two dimensional gel electrophoresis.