Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition Nov. 2015

Study of Drug Resistance in Salmonella spp. Isolated from Native Eggs of Iran?s Southern Region

Ali Ghorbani Ranjbary1, Hossein Kargar Jahromi2*, Nazanin Ghorbani Ranjbary1, Sheyda Asmarian3, Fatemeh Juibar1 and Navid Kalani2

1Young Researchers and Elite Club, Kazerun Branch, Islamic Azad University, Kazerun, Iran. 2Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran. 3Departement of Phamacology, Faculty of Veterinary, Kazerun Branch, Islamic Azad University, Kazerun, I.R. Iran.

Received on 25 April 2015 and accepted on 21 July 2015

 

ABSTRACT

Salmonellas are short bacteria, gram negative, some of them have caps, facultative aerobic and anaerobic have high resistance against physical and chemical factors. Salmonella causes bacillary white diarrhea in Avian. Its transference in Avian is vertical and horizontal. In human, it causes food poisoning too. The egg is the most important source of salmonella. Native eggs have been collected and transferred to the lab in order to study their contamination. These eggs were collected from areas around Shiraz (Iran). In this research the pattern of drug resistance of bacteria was studied. The egg shell is purified with Ethanol 70%, and then the contents of Fifty eggs are mixed in a dish and incubated with soab in Selenite-f. After 24 hour incubation at 37?C the Selenite-f sample was transferred to salmonella spp and after incubation at 37?C was analyzed in terms of suspected colonies to Salmonella. The suspected colonies were inoculated into lysine decarboxylase broth and TSI agar environment. The bacteria that had reactions were related to salmonella, and were analyzed by the PCR test, with special primer for salmonella spp, such as S. enteritidis and S. typhimurium. The results of this study indicated five samples of mixed eggs (at least 0/33% of eggs) were contaminated with salmonella of serotype S. enteritidis. Among the separated Salmonellas 85.9 % were resistant to Ampicillin, 14.5% to Tetracycline, and 42.9% to Kanamycin, but all of them were sensitive to Norfloxacin in this antibiotic resistance test. Excessive use of antibiotics and also having no information on sensitivity and bacterial resistance to antibiotics can make the antibiotic resistances more complex.

Keywords : Salmonella spp., Salmonella enteritidis, Drug resistance.