Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Effect of Trichoderma harzianum on Meloidogyne javanica in Tomatoes as Influenced by Time of the Fungus Introduction into Soil

Muhammad TariqJaveed* and Ahmad Saad Al-Hazmi

Department of Plant Protection, King Saud University, P.O Box 2460 Riyadh 11451, Saudi Arabia.

Received on 07 February 2015 and accepted on 10 March 2015



A greenhouse pot experiment was conducted to determine the effect of time of introduction of Trichoderma harzianum on its biocontrol efficacy against Meloidogyne javanica in tomatoes. The experiment consisted of all possible combinations of individual, concomitant and sequential inoculations of T. harzianum and M. javanica. Results indicated that time of the fungus introduction into the soil proved to be important. Introduction of T. harzianum to M. javanica-infested soil at or prior (one or two weeks) to nematode inoculation suppressed the nematode reproduction and increased host growth. However, such effects were increasingly greater when the fungus was introduced two or one week prior to nematode inoculation.

Keywords : Antagonistic effect, Application time, Biological control, Nematophagous fungi, Root-knot nematodes, Solanum lycopersicum.