Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Effects of Pesticides and Respiratory Inhibitors on Phenol Degradation by Acinetobacter sp. strain AQ5NOL 1 Immobilized in Gellan Gum

Siti Aqlima Ahmad1*, Mohd Yunus Shukor1, Nor Aripin Shamaan2, Nor Arina Ab Rahman1, Farrah Aini Dahalan3, Khalilah Abdul Khalil4 and Mohd Arif Syed1

1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia. 2Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 13th Floor, Menara B, Persiaran MPAJ, Jalan Pandan Utama, Pandan Indah, 55100 Kuala Lumpur, Malaysia. 3The School of Environmental Engineering, Universiti Malaysia Perlis, Kompleks Pengajian Jejawi 3, 02600 Arau, Perlis. 4Department of Biomolecular Science, Faculty of Applied Sciences, Universiti Teknologi MARA, Seksyen 2, 40450 Shah Alam, Selangor, Malaysia.

Received on 02 January 2015 and accepted on 03 March 2015

 

ABSTRACT

Phenol pollution often associated with agriculture industries involving pesticides and respiratory inhibitors. Microorganism used for biodegradation of phenols should also be resistant to the pesticides and respiratory inhibitors. In this study, 1 ppm of carbofuran, paraquat dichloride, atrazine, potassium cyanide (KCN), sodium azide (NaN3) and rotenone were used to investigate the ability of Acinetobacter sp. strain AQ5NOL 1 freely suspended and immobilized in gellan gum beads to degrade phenol in the presence of pesticides and respiratory inhibitors. Results from this study showed that the degradation of phenol after 48 hours of incubation by free cells was inhibited by KCN at 47.48%. However, the degradation of phenol after 18 hours incubation by immobilized cells was inhibited by KCN at 52.68%. The lowest concentration of KCN that showed inhibition to phenol degradation was 0.8 ppm. Prolonging the incubation time from 18 hours to 20 hours for KCN has alleviated the inhibition. Other respiratory inhibitors such as carbofuran, paraquat dichloride, atrazine, NaN3 and rotenone showed no effect on phenol-degrading activities and bacterial growth by both free and immobilised cells compared to control (p>0.05).

Keywords : Immobilised cell, phenol degradation, pesticides, respiratory inhibitors, Acinetobacter sp.