Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Genotypic Characterization of Buffalo Isolates of Echinococcus granulosus

A. Sheeba*, A. Sangaran, B.R. Latha and A. Raja

Department of Veterinary Parasitology, Madras Veterinary College, Chennai - 600 007, India.

Received on 18 February 2015 and accepted on 12 April 2015

 

ABSTRACT

Cystic echinococcosis (CE) caused by the larval stage of the cestode Echinococcus granulosus is one of the most widespread zoonoses of veterinary and medical importance. Molecular techniques have allowed the identification of 10 different genotypes (G1-G10)of the parasite. Numerous studies have provided evidence that Echinococcus granulosus exists as a complex of different strains, that differ in wide variety of criteria that have impact on the epidemiology, pathology and control of cystic hydatid disease (CHD). On sequence analysis of mitochondrial COX1 and NADH genes showed the occurrence of the G1 genotype, the common sheep strain, G2 genotype, the Tasmanian sheep strain, the G3 genotype, the buffalo strain. The present study is an update of E.granulosus strains by genotyping a large samples of isolates and checking out genetic differentiation within and among the G1,G2,G3 genotype using additional gene marker, the RRNS gene. Sequencing of RRNS gene revealed fixed nucleotide substitution among G1,G2 and G3 genotypes. This study provides further evidence of the occurrence of the E.granulosus G1 and G2 sheep strain.

Keywords : Echinococcus granulosus ; Genotypes; Mitochondrial DNA genes.