Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Influence of Growth in Biofilm in the Formation of New Biofilm by Clinical Isolates of Acinetobacter baumannii

Mohammed S. Al-Mogbel1,2, Mushtaq Khan1,2, Mamoun Shadeed1, Mamdoh Meqdam1 and Ashfaque Hossain2,3*

1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, 2Molecular Diagnostics and Personalized Therapeutic Unit, 3Department of Microbiology, College of Medicine, University of Ha’il, Ha’il, Saudi Arabia.

Received on 03 January 2015 and accepted on 06 March 2015

 

ABSTRACT

The influence of growth in biofilm in formation of new biofilm by clinical isolates of Acinetobacter baumannii was investigated in this study. Sequential passage of A.baumannii isolates in biofilm culture in trypticase soy broth (TSB) resulted in gradually increased amount of biofilm production the by the isolates. On the other hand, passage of the same isolates in planktonic culture did not result in enhanced biofilm production. Passage induced enhanced biofilm production reached maximum level at passage 3 (P-3) for the strains A.baumannii strains AB-1 and AB-3 and passage 4 (P-4) for the strain AB-2. These values were 47 %, 40.4% and 71.8 % increased biofilm production, respectively by the biofilm passaged strains in comparison to their planktonic counterparts. Normal human serum (NHS), which enhances biofilm production by A.baumannii isolates was investigated to determine its effect on passage induced biofilm formation. NHS was found to further increase biofilm production by A.baumannii strains grown in biofilm mode, in comparison to their counterparts grown in planktonic state. Taken together, the results of this study indicate that growth of A. baumannii in biofilm enhances its potential to form new biofilm and biofilm formation is further increased by the presence of NHS in the growth medium.

Keywords : Acinetobacter baumannii, Biofilm, Normal human serum, Virulence, Infection.