Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Micro Fibril and Capsular Exopolysaccharides Produced by Alcaligenes CMG634

Nazia Jamil1* and Nuzhat Ahmed2

1Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore-54590, Pakistan. 2Centre for Molecular Genetics, University of Karachi, Karachi-75270, Pakistan.

Received on 11 January 2015 and accepted on 20 March 2015

 

ABSTRACT

An attached green seaweed bacteria CMG634 was isolated from Gaddani beach of Karachi coast (around 25.5oN, 66.7oE). It produced translucent and gelatinous colonies on agar plates. Strain CMG634 (AY596795) was identified as Alcaligenes sp. by 16S ribosomal RNA. Exopolysaccharide was analyzed by extracting the samples of broth at different intervals. When the isolated bacterium was grown at 30oC for a few days (1-5) on 0.5M NaCl nutrient broth. Partial purification of the extracted polysaccharide was done by dissolving of the precipitates in water and extracted by ethanol. The partially purified polysaccharide was subjected to acid hydrolysis and neutral fractions were analyzed by thin layer chromatography. Extracted polymer was found to be Glycoprotein in nature. Hygroscopic analysis of extracted EPS showed that maximum water was absorbed by EPS of 5th day. Scanning and transmission electron micrographs showed both capsular and slime in form of micro fibril.

Keywords : Exopolysaccharides, 16S ribosomal RNA gene, thin layer chromatography, bioabsorbant, micro fibril,transmission electron micrographs.