Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Comparison and Optimization of Biological and Antibacterial Activity of Indian White Shrimp Waste Extracted by Biosynthesis Methods through Response Surface Methodology

Marjan Nouri1, Faramarz Khodaiyan1*, Seyed Hadi Razavi2 and Mohammad Ali Mousavi2

1Bioprocessing and Biodetection Laboratory, Department of Food Science, Engeneering and Technology, Faculty of Agricultural Engineering and Technology, Campus of Agriculture and Natural Resource, University of Tehran, P.O. Box 4111, Karaj 31587-77871, Iran. 2Department of Food Science, Engineering and Technology, Faculty of Agricultural Engineering and Technology, Campus of Agriculture and Natural Resources, University, Tehran, P.O. Box 4111, Karaj 31587-77871, Iran.

Received on 06 Feburary 2015 and accepted on 11 March 2015

 

ABSTRACT

Indian white shrimp waste extracted by biosynthesis methods involving demineralization, deproteinization and deacetylation. In this study chitosan with high functionality was produced using mild conditions (temperature (60, 80 and 100 C), concentration of alkaline (30, 40 and 50 %) and time (90, 195, 300 mins) of reaction in chemical method and power of microwave (300, 600 and 900 W), concentration of alkaline (30, 40 and 50 %) and irradiation time (20, 100, 180 S) in microwave method). Results showed that significant differences (P<0.05) were observed in decreasing of the viscosity and molecular weight of the chitosan samples with increasing concentration of alkali solution (30 to 50 %). DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activities of chitosan samples (15.26 to 17.77%) were observed and samples had low antioxidant activity compared with BHT (Butylated hydroxyltoluene). The antibacterial activities were examined against bacteria (Escherichia coli, Salmonella typhimurium and Staphylococcus aureus). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of chitosan showed the inhibition of the growth of various bacteria tested although it depends on the molecular weight of samples and the species of bacteria.

Keywords : Indian white shrimp, Chitosan, Biosynthesis, Antimicrobial activity, Response surface methodology (RSM).