Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Metabolomic Investigation of Elizabethkingia meningoseptica Response Challenge with Excoecaria agallocha Leaf Extract

A.A. Laith1,2*, A.G. Mazlan1, T.S. Tengku Muhammad3, L.K. Teh4, M.Z. Salleh4 and M. Najiah1

1School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia. 2Institute of Tropical Aquaculture (AQUATROP), Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia. 3Institutes of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia. 4Integrative Pharmacogenomics Institute, UniversitiTeknologi MARA, 42300 Bandar PuncakAlam, Selangor, Malaysia.

Received on 18 January 2015 and accepted on 11 March 2015

 

ABSTRACT

Disease causes alterations in metabolism that may possibly be acknowledged as metabolic signatures and used to distinguish healthy and disease conditions.The present study was conducted for analytical platform that allows comprehensive analysis of microbial metabolomics. Different metabolites have been evaluated in vivo to characterize Elizabethkingia meningoseptica response on effects of methanol leaf extracts of Excoecaria agallocha using Liquid chromatography/mass spectrometry (LC/MS), utilizing a time-of-flight (TOF) mass analyzer. In vitro screening at different concentrations of E. agallochamethanol leaf extracts showed alteration in the shape of E.meningoseptica using scanning electron microscope (SEM). The profile of metabolites in vivo experiment showed 82 compounds that were initially identified.Only 75 compounds satisfy the P-Corrcut-off point of less than 0.05 and at least 2-fols change. In bacteria isolated from skin and kidney, initially a total of 240 metabolites were identified and only 79 metabolites satisfied the P-Corrcut-off point of less than 0.01 and at least 2-fold change. The result from scanning electron microscope (SEM) showed E.meningoseptica destroyed at concentration of 12.5 mg/mlof methanol leaf extracts of E. agallocha. To conclude, E. agallocha are diverse, produce compounds with antimicrobial activity and are a suitable sourcefor antimicrobial natural products.

Keywords : Elizabethkingia meningoseptica, metabolites, LC/MS Q-TOF,Excoecaria agallocha,SEM.