Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Aqueous two-phase Extraction of Intracellular Lipase with Sn-1,3 Positional Selectivity from A. niger GZUF36

Chao-Shuang Xia1,2, Yu-Di Wu1,2, La-Ping He1,2,3*, Fu-Hao Zhang1,3, Cui-Qin Li4, Yi-Ming Zhang1 and Li Chen1

1College of Liquor and Food Engneering, Guizhou University, Guiyang 550025, China; 2Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang - 550025, China. 3Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang - 550025, China. 4School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.

Received on 16 February 2015 and accepted on 20 April 2015

 

ABSTRACT

The extraction of intracellular lipase with Sn-1,3 positional selectivity from a new strain of A. niger GZUF36 has been exploited by aqueous two-phase system (ATPS). Firstly, crude intracellular lipase solution was prepared by cell disruption. Sonication is the best method for disruption of A. niger cell. Then, extraction of the intracellular lipase from the crude enzyme solution was focused on by ATPS. The influence of key parameters such as molecular weight of polyethylene glycol (PEG), PEG 2000 concentration and (NH4)2SO4 concentration on the partitioning behavior of lipase was evaluated. The suitable aqueous two-phase system consisted of 20% PEG 2000 and 12% (NH4)2SO4 resulted in one-sided partitioning of the intracellular lipase with selectivity of 2.39, recovery of lipase 74.93% and purification factor of 3.42-folds. ATPS extraction may be used as a first step to purify the enzyme from crude enzyme solution.

Keywords : Sn-1,3 position selectivity; intracellular lipase; A. niger GZUsF36; aqueous two phase system; extraction.