Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Isolation and Molecular Identification with Resistant Profile Determination of Listeria monocytogenes from Imported Chicken Carcasses in Duhok, Kurdistan Region, Iraq

Meqdad S. Ahmed, Zanan M.A. Taha and Lokman T. Omer

University of Duhok, Faculty of Veterinary Medicine, Duhok Veterinary Research Center (DVRC), Duhok, Iraq.

Received on 20 January 2015 and accepted on 06 March 2015

 

ABSTRACT

This study was carried out to determine the contamination rate of L. monocytogenes from imported chickens in Duhok, Kurdistan region of Iraq and to determine their susceptibility profiling to different antibiotics. A total chickens (100 samples) were swab sampled, examined by conventional cultural methods and confirmed by PCR technique. Out of 100 only 3 (3%) chickens were found to be contaminated with L. monocytogenes. (100%) of isolates were resistant to Ceftazidime, and Ceftriaxone, (66.6%) to Ciprofloxacin and Clindamycin, while (100%) were susceptible to each of Imipenem, Levofloxacin, Ticarcillin-calvulanic acid, Clarithromycin and Gentamicin. The presence of this pathogen with an MDR profile on chicken carcasses found in this study creates a major risk for the consumer and need good hygienic strategies.

Keywords : L. monocytogenes; chicken carcass; isolation, molecular identification; resistant profiling.