Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Molecular Identification and Characterization of Phenol - Degrading Bacteria from Oil-Contaminated Sea Water

Hend Abdulhameed Hamedo1*, Aliaa Ragab El-Shamy2 and Hassan Mohamed Awad2

1Faculty of Science, El Arish, Suez Canal University. 2Chemistry of Natural and Microbial Products Dept., Pharmaceutical Industries Div., National Research Centre, 33 EL Bohouth St., Dokki, P.O.Box:12622, Giza, Egypt.

Received on 25 March 2015 and accepted on 03 May 2015

 

ABSTRACT

Biological treatment is one of the considerable choices for removing of phenol as a main pollutant present in the environment. Recently phenol biodegradation has been considered, and marine bacteria are the most important phenol biodegrader. In this study, the phenol degrading bacteria from marine environmental samples were isolated from RasGhareb Red Sea Governorate, Egypt. Isolation and identification of the most effective bacterial species using direct isolation method on mineral salt medium supplemented with 2.0 mM phenol as the sole carbon source are mentioned. The two phenol-degrading bacteria were characterized by morphological, biochemical properties and confirmed by DNA fingerprinting technique. Ribosomal database project consistent with phylogenetic analysis of 16S rRNA gene sequences showed that the strain RGRS-1 has 99% similarity with Ochrobactrum sp. strains while RGRS-2 has 98% similarity with Kocurica camiphilia strains. The DNA G+C content of strain RGRS-1 was 53 mol% while RGRS-2 was 57 mol%. On the basis of polyphasic analysis, strain RGRS-1 is considered to represent a novel species of the genus Ochrobactrum, for which the name Ochrobactrum sp. RGRS-1 is proposed. On the other hand, RGRS-2 proposed a name is Kocurica camiphilia RGRS-2 .The nucleotide sequences of 16S rRNA gene have been deposited in GenBank under accession numbers: KP221808 and KP221809 for RGRS-1 and RGRS-2 respectively. The aerobic isolates grew on phenol at concentrations ranging from 2-10 mM. The ability of the isolates to degrade phenol make them a potential candidate for use in bioremediation of environments contaminated by such or related compounds.

Keywords : Biodegradation, Bioremediation, marine water, phenol, Ochrobactrum sp. RGRS-1, Kocurica camiphilia RGRS-2.