Journal of Pure and Applied MicrobiologyVol. 9 No. 1

Comparative Study of Dark Field Microscopical Examination, Microscopic Agglutination Test and Polymerase Chain Reaction in the Diagnosis of Bovine Leptospirosis

G. Balakrishnan, Parimal Roy and T.V. Meenambigai

Department of Veterinary Microbiology, Madras Veterinary College, Chennai - 600 051, India.

Received on 06 Augsut 2014 and accepted on 29 October 2014



A total of 115 serum samples (33 from cattle and 82 from buffaloes) collected from different clinical cases (65 Nos) like abortion, repeat breeding, jaundice and haemorrhagic mastitis and also from apparently healthy bovines were subjected to Dark field microscopical examination, Microscopic agglutination test and Polymerase chain reaction to diagnose bovine leptospirosis. The present study showed high positivity in MAT (58.46 %) followed by DFM (38.46 %) and PCR (32.31 %). The study concluded to use MAT for serosurveillance study and MAT followed by PCR for the diagnosis of leptospirosis.

Keywords : Leptospira, DFM, MAT, PCR, Diagnosis.