Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Population study of African catfish Clarias gariepinus in India using microsatellite DNA

P. Ezilrani1, J. Godwin Christopher1* and K. Karal Marx2

1Molecular and Microbiology Research Laboratory, Environmental Biotechnology division, School of BioSciences and Technology, VIT University, Vellore-632014, India. 2Fisheries biotechnology Centre, Department of Aquaculture, Fisheries College and Research Institute, Thoothukudi-628008, India.

Received on 08 January 2015 and accepted on 24 March 2015

 

ABSTRACT

Genetic variation between six populations of Clarias gariepinus were analyzed using six microsatellite loci. All the loci were highly polymorphic among all samples. The mean number of alleles and effective alleles per locus ranged from 4.667 to 5.833 and 2.000 to 6.000 respectively. The heterozyosities observed were found to be 0.739Ī0.012. Conformity to Hardy Weinberg Equilibrium using chi-square test showed that at 26 test out of 36 test (P<0.005) showed a significant value. The population differentiation (FST= -0.0170) was lower than gene flow (Nm= 0.8885) indicating heterozygosities in the population. The polymorphism information content (PIC) were greater than 0.6 in most of the loci indicating informative marker for the selected loci. The Neiís genetic distance was high between Pop1 and Pop6 with 5.669. The observations showed that due to allelic diversity and estimates of heterozygosity, these markers can be useful in the genus Clarias for population level analysis in the Indian Clariidae fishes using microsatellite DNA markers.

Keywords : Genetic variability, Polymorphism , Genetic distance , Neutrality, AMOVA