Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Vaginal Probiotics and Invitro Inhibition of Herpes Vaginal Infection

Reza Ranjbar1, Mehdi Moazzami Goudarzi2* and Nematollah Jonaidi3

1Molecular Biology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran. 2Department of Microbiology, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran. 3Health Research Center, Baqiyatallah University of medical science, Tehran, Iran.

Received on 20 March 2015 and accepted on 30 April 2015

 

ABSTRACT

The protective effect of selected vaginal Lactobacillus strains (L. brevisCD2, L. salivarius FV2, L. plantarum FV9)towards herpes simplex virus type 2 (HSV-2) infection in vitrohas been analyzed. Living bacterial cells affect differentsteps of virus multiplication. The effect on the early phases of virus infection appeared related to the bacterial adhesivepotential to the cell membrane while all the strains strongly reduced intracellular events of virus multiplication. The antiHSV-2 activity was not mediated by a virucidal effect. Instead it was exerted through bacterial soluble factors able todown regulate the production of infective virions. In fact HSV-2 yield was significantly reduced in infected cells fedwith cell-free supernatants of lactobacilli grown in cell culture medium. Purified lactic acid and H2O2, Lactobacillusmetabolites with known antimicrobial activity, produced a dose-dependent virucidal effect. Lactic acid successfullyinterfered with viral intracellular antigen synthesis and both the virucidal activity and the inhibition of replication werecorrelated to acidic pH values. L. brevis CD2, the most active strain, does not produce H2O2and neutralized lactic acidhad no effect, thus indicating that factors other than H2O2and lactic acid could be responsible for the antiviral effect.

Keywords : Herpes simplex virus type 2, Vaginal lactobacilli.