Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Biosurfactant Production by Bacterial Isolates from Marine Sediments

M.D. Dipti, I. Ankita, B. Monalisa, D. Anamika and W.J. Osborne

School of Bioscience and Technology, VIT University, Vellore- 632014, India

Received on 04 October 2014 and accepted on 25 November 2014

 

ABSTRACT

The application of detergents in the fabrics leads to pollution to the environment and causes harmful effect to aquatic organism. This study aims at using surfactants produced by bacteria to curb the hurdles posed by use of synthetic detergents. In the present study bacterial isolates were obtained from marine mangrove sediments collected from Rameshwaram coast, which harbours diverse population of microorganisms. The isolates were found to be gram negative. Two isolates were assessed for the ability to produce biosurfactants. After fermentation in MSM media supplemented with glycerol fabric assay was performed and the isolates VITDAMJ 1 and 2 was found to be capable of producing biosurfactants. The effective isolates were characterized by 16S rRNA sequencing.

Keywords : Enterobacteriaceae and Fabric assay