Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Characterization of Growth and Bacteriocin Production by Lactococcus lactis sub sp. lactis R10 Isolated from Fermented Radish

P. Umamakesh* and K. Vijila

Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India.

Received on 30 August 2014 and accepted on 25 October 2014

 

ABSTRACT

In the present study, the kinetics of growth and bacteriocin production by a Lactic acid bacterial (LAB) strain obtained from fermented radish was studied. The growth of the strain showing a short lag phase of 2 h and steady increase up to 24 h, attained maximum growth at stationary phase. The LAB strain produced maximum bacteriocin activity of 644.00 AU ml-1 at stationary phase of growth. The bacteriocin production of the strain under varied physiological conditions, such as pH, temperature and NaCl were evaluated. The maximum growth and bacteriocin production was observed at 6.5 pH and 37?C. Slight improvement in bacteriocin production was noted, when MRS broth was supplemented with 2% NaCl. The potential bacteriocin producing LAB strain was identified as Lactococcus lactis subsp. lactis R10 using 16S rRNA gene sequence.

Keywords : Bacteriocin, Physiological characterization, Lactococcus lactis subsp. lactis R10.