Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Optimization of Growth Parameters for Elevated Production of Mycoprotein - Fusarium venenatum using Response Surface Methodology

P. Prakash, S. Karthick Raja Namasivayam* and G. Narendrakumar

Department of Biotechnology, Sathyabama University, Chennai - 600 119, India.

Received on 18 August 2014 and accepted on 23 October 2014

 

ABSTRACT

Fusarium venenatum ? Mycoprotein are rich in protein, sugars and essential fatty acid and supplemented as dietary food for human and animal consumption in the various parts of the world. Biomass production is highly prejudiced by supplying nutritional factors in the media. Response surface methodology (RSM) is an important tool to optimize the various essential factors for the production of biomass and yield the desired concentration of metabolite that satisfy the requirement in bioprocess industries. In the present study, Response surface methodology via Central composite Design (CCD) was used to optimize the medium components to improve biomass yield. The medium components involved are liquefied jaggery, date extract, KH2PO4 and K2HPO4. Thus maximum biomass was observed in 7th trial, the medium components were liquefied jaggery 2.5 ml, date extract 8ml, KH2PO4 500mg, K2HPO4 100mg and 5% of inoculum with 72 hrs.

Keywords : Fusarium venenatum , Mycoprotein, Response surface methodology (RSM), biomass.