Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Bio-prospecting of Root Endophytic Aquatic Fungus Cylindrocarpon aquaticum (Nils.) Marvanova and Descals as Antibacterial Potential

Lokendra Singh* and S.C. Sati

Department of Botany, DSB Campus, Kumaun University, Nainital 263 002, India

Received on 25 November 2013 and accepted on 08 February 2014

 

ABSTRACT

Root endophytic freshwater hyphomycetes Cylindrocarpon aquaticum (Nils.) Marvanova and Descals was isolated and evaluated for its antimicrobial potential against some Gram-positive (Bacillus subtilis) and Gram-negative (Agrobacterium tumefaciens, Escherichia coli, Erwinia chrysanthemum, Xanthomonas pseudomonas) pathogenic bacterial strains. Antimicrobial activity was assessed with preliminary and secondary antimicrobial assays. The results were found positive with the isolated root endophytic fungus and a significant zone of inhibition was observed for all tested bacterial strains. In preliminary antimicrobial assay maximum zone of inhibition was counted against Gram-positive bacterium Bacillus subtilis (26 mm) followed by Gram-negative bacteria Xanthomonas phaseoli (24 mm) and Agrobacterium tumefaciens (23 mm) while minimum zone of inhibition was observed against Erwinia chrysanthemi and Escherichia coli (22 mm, each). Whereas, in secondary antimicrobial assay the maximum zone of inhibition was recorded against human pathogenic bacterial strain Escherichia coli (16 mm) followed by Bacillus subtilis (15 mm) and minimum inhibition was found against Agrobacterium tumefaciens (10 mm).

Keywords : Cylindrocarpon aquaticum, root endophyte, aquatic fungus, antimicrobial potential, pathogenic bacteria.