Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Lipid Biosynthesis and Fatty acid Characterization in Selected Spirulina Strains under Different Cultivation Systems

R.N. Bhakar1, R. Kumar1,2, S. Elumalai2 and Sunil Pabbi1*

1Centre for Conservation and Utilisation of Blue Green Algae (CCUBGA), Division of Microbiology, Indian Agricultural Research Institute, New Delhi - 110012, India. 2Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai - 600005, India.

Received on 30 August 2014 and accepted on 29 October 2014



Total lipids production and fatty acid profile in Spirulina strains cultivated in flasks in a culture room and in photobioreactor under optimized set of culture conditions revealed that production of total lipid was more under photobioreactor conditions (23.64%) than in flasks (17.72%). Although similar fatty acids could be detected under both cultivation conditions but their amounts varied considerably. The percentage ratio of gamma-linolenic acid (GLA) to total fatty acids (TFA) was found upto 26.83% and 28.20% under flask and photobioreactor conditions respectively and the PUFA content was enhanced upto 71.83 - 82.60% and 77.02 ? 83.03% of total fatty acids for flask and photobioreactor conditions respectively.

Keywords : Spirulina, Photobioreactor, Fatty acids, Palmitic acid, Linoleic acid, g -linolenic acid.