Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Liquid Formulation of Azotobacter chroococcum (ABA-1) for Seed Inoculation

H.N. Shelat*, P.K. Acharya, Y.K. Jhala and R.V. Vyas

Department of Microbiology & Biofertilizer Project, B.A.College of Agriculture, Anand Agricultural University, Anand, Gujarat - 388110, India.

Received on 20 August 2014 and accepted on 30 October 2014



Liquid, Carrier and Granular formulations of Azotobacter chroococcum (ABA-1) were prepared from Jensen?s broth having 9.5x1011 cfu/ml . Bacterial survival in formulations as well as on inoculated Pearl millet seeds was studied using Jensen?s agar and there in vivo efficacy testing on growth parameters of P.millet cv GHB-558 in pots. In formulations, Azotobacter count was decreased 10 and 100 folds after 3 and 5 months, respectively. Survival of bacteria on seed was reduced from 1010 to 105 after 48 hours. Seed germination effect of all formulations on seed agar showed increased root and shoot length with secondary root formation as compared to uninoculated control. In a pot trial, Azotobacter formulation containing 2% glycerol + 2% PVP registered highest plant height, shoot and root weight at 60 DAS by seed application in Pearl millet , which was significantly superior over other formulations, recommended dose (80 kg ha-1) of nitrogen and absolute control.

Keywords : Azotobacter, Liquid formulation, Seed application, Pearl millet.