Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Assessing the Establishment of Lac Z Marked Strains of Pentachlorophenol Degrading Rhizobacteria and their Effects on Plant Growth of Maize (Zea mays)

K.M. Avitha1, K.S. Jagadeesh1, P.U. Krishnaraj2 and Sarita Sinha3

1Department of Agricultural Microbiology, University of Agricultural Sciences, Dharwad - 580 005, India. 2Department of Biotechnology, University of Agricultural Sciences, Dharwad - 580 005, India. 3Ecotoxicology and Bioremediation Group, National Botanical Research Institute, Lucknow - 226 001, India.

Received on 06 April 2014 and accepted on 19 August 2014

 

ABSTRACT

Rhizobacteria isolated from the rhizosphere of PCP tolerant maize plant were investigated for establishment of lac Z marked strains of PCP degrading bacteria in the rhizosphere of maize (Zea mays) under green house condition. The identities of the isolates were determined by 16S rRNA gene sequencing as Enterobacter asburiae (MAZ1) and Pseudomonas (MAZ2).?Both the lac Z marked strains were able to survive in rhizosphere of maize under greenhouse conditions and maintained a population of about 104?g?1?of rhizosphere soil up to 60 days study period. Positive effect of inoculation with PCP degrading bacteria on plant length and biomass were observed. Out of two, PCP degrading Pseudomonas sp. MAZ 2 was found to possess higher root colonization ability and plant growth promoting potentials.

Keywords : PCP degrading, rhizobacteria, lac Z marked strains, Maize.