Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Assessment of Antimicrobial Activity of Biogenic Silver Nanoparticles against Plant Pathogens

Aradhana Sukhwal1, Arunabh Joshi1, Pokhar Rawal2 and Devendra Jain1*

1Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur - 313001, India. 2Department of Plant Pathology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur - 313001, India.

Received on 16 September 2014 and accepted on 30 October 2014

 

ABSTRACT

With the ever increasing demand of the crop production, technology calls for innovative ideas for better disease management leading to higher crop production with available resources. Multiple diseases caused by plant pathogens affect plant crops leads to major losses up to 30% and simultaneously decreasing the quality and safety of agricultural products. The silver nanoparticles used in the present study were prepared through a cost effective and environment friendly green synthesis technique by using leaf extract of Tagetes patula L. (french marigold) as reducing as well as capping agent. The prepared biogenic nanoparticles were well characterized using U.V. visible spectroscopy, Transmittance electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy which revealed the size of nanoparticles ranging from 15-30nm as well revealed their cubic and hexagonal structure. Further these biologically synthesized nanoparticles were found to be highly toxic against phytopathogenic bacteria and fungi by poison food technique, disc diffusion method and spore germination inhibition test.

Keywords : Green synthesis, Silver nanoparticles, Food poisoning method, Disc diffusion method, Spore germination inhibition method.